Virus:Article Title: Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP Rockland Immunochemicals Cat#600-401-379S; RRID: AB_11182807 Chicken anti-GFP Aves Labs Cat#GFP-1010; RRID: AB_2307313 Guinea pig anti-VGlut Millipore Cat#AB5905; RRID: AB_2301751 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat#A-11011 Alexa Fluor 488 goat anti-chicken Invitrogen Cat#A-11039 Alexa Fluor 568 goat anti-guinea pig Invitrogen Cat#A-11075 Bacterial and virus strains AAV-EF1ɑ-Cre-mCherry UNC Viral Core N/A AAV-PhP.eb-CMV-sadCas9-VP64 Tamura et al.52 N/A AAV-PhP.eb-U6-sasgRNA-CMV-mCherry Tamura et al.52 N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris Cat#2312 Picrotoxin Tocris Cat#1128 CPCCOEt Tocris Cat1028 Pilocarpine hydrochloride Sigma-Aldrich Cat#P6503-5G Phosphate buffered saline Sigma-Aldrich Cat#SIAL-P4417-100TAB Paraformaldehyde VWR International Cat#100504-858 Sodium azide Sigma-Aldrich Cat#S2002-100G Triton X-100 Sigma-Aldrich Cat#X100-100ml Normal goat serum Fisher Scientific Cat#NC9660079 ProLong Gold with DAPI Life Technologies Cat#P36931 Vybrant DiI Cell-Labeling Solution Invitrogen Cat#V22885 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory JAX Strain #:000664; RRID: IMSR_JAX:000664 Mouse: Scn2a+/- Planells-Cases et al.80 N/A Mouse: Alpha6 - Cre Mutant Mouse Recourse & Research Centers RRID: MMRRC_015966-UCD Mouse: Scn2a+/fl Spratt et al.16 N/A Software and algorithms IGOR Pro Wavemetrics RRID: SCR_000325; v6.3 Prism Graphpad RRID: SCR_002798; v9 LabView NI RRID: SCR_014325; v2020 MATLAB MathWorks RRID: SCR_001622; vR2020a DeepLabCut Mathis et al.81 RRID: SCR_021391; v2.2.0.5 KiloSort2 Pachitariu et al.82,83 RRID: SCR_016422; https://github.com/ MouseLand/Kilosort Phy Cyrille Rossant https://github.com/cortex-lab/phy Mouse eye tracking acquisition code in LabView Liu et al.84; Bouvier et al.83 N/A Visual stimuli code in MATLAB Liu et al.84 N/A Human eye tracking acquisition code Dave Jones https://github.com/waveform80/picamera Human motion tracking (IMU) acquisition code This paper https://doi.org/10.5281/zenodo.10573756 Neuron 112, 1–12.e1–e5, May 1, 2024 e1 ..
Bioprocessing:Article Title: Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP Rockland Immunochemicals Cat#600-401-379S; RRID: AB_11182807 Chicken anti-GFP Aves Labs Cat#GFP-1010; RRID: AB_2307313 Guinea pig anti-VGlut Millipore Cat#AB5905; RRID: AB_2301751 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat#A-11011 Alexa Fluor 488 goat anti-chicken Invitrogen Cat#A-11039 Alexa Fluor 568 goat anti-guinea pig Invitrogen Cat#A-11075 Bacterial and virus strains AAV-EF1ɑ-Cre-mCherry UNC Viral Core N/A AAV-PhP.eb-CMV-sadCas9-VP64 Tamura et al.52 N/A AAV-PhP.eb-U6-sasgRNA-CMV-mCherry Tamura et al.52 N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris Cat#2312 Picrotoxin Tocris Cat#1128 CPCCOEt Tocris Cat1028 Pilocarpine hydrochloride Sigma-Aldrich Cat#P6503-5G Phosphate buffered saline Sigma-Aldrich Cat#SIAL-P4417-100TAB Paraformaldehyde VWR International Cat#100504-858 Sodium azide Sigma-Aldrich Cat#S2002-100G Triton X-100 Sigma-Aldrich Cat#X100-100ml Normal goat serum Fisher Scientific Cat#NC9660079 ProLong Gold with DAPI Life Technologies Cat#P36931 Vybrant DiI Cell-Labeling Solution Invitrogen Cat#V22885 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory JAX Strain #:000664; RRID: IMSR_JAX:000664 Mouse: Scn2a+/- Planells-Cases et al.80 N/A Mouse: Alpha6 - Cre Mutant Mouse Recourse & Research Centers RRID: MMRRC_015966-UCD Mouse: Scn2a+/fl Spratt et al.16 N/A Software and algorithms IGOR Pro Wavemetrics RRID: SCR_000325; v6.3 Prism Graphpad RRID: SCR_002798; v9 LabView NI RRID: SCR_014325; v2020 MATLAB MathWorks RRID: SCR_001622; vR2020a DeepLabCut Mathis et al.81 RRID: SCR_021391; v2.2.0.5 KiloSort2 Pachitariu et al.82,83 RRID: SCR_016422; https://github.com/ MouseLand/Kilosort Phy Cyrille Rossant https://github.com/cortex-lab/phy Mouse eye tracking acquisition code in LabView Liu et al.84; Bouvier et al.83 N/A Visual stimuli code in MATLAB Liu et al.84 N/A Human eye tracking acquisition code Dave Jones https://github.com/waveform80/picamera Human motion tracking (IMU) acquisition code This paper https://doi.org/10.5281/zenodo.10573756 Neuron 112, 1–12.e1–e5, May 1, 2024 e1 ..
Recombinant:Article Title: Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP Rockland Immunochemicals Cat#600-401-379S; RRID: AB_11182807 Chicken anti-GFP Aves Labs Cat#GFP-1010; RRID: AB_2307313 Guinea pig anti-VGlut Millipore Cat#AB5905; RRID: AB_2301751 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat#A-11011 Alexa Fluor 488 goat anti-chicken Invitrogen Cat#A-11039 Alexa Fluor 568 goat anti-guinea pig Invitrogen Cat#A-11075 Bacterial and virus strains AAV-EF1ɑ-Cre-mCherry UNC Viral Core N/A AAV-PhP.eb-CMV-sadCas9-VP64 Tamura et al.52 N/A AAV-PhP.eb-U6-sasgRNA-CMV-mCherry Tamura et al.52 N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris Cat#2312 Picrotoxin Tocris Cat#1128 CPCCOEt Tocris Cat1028 Pilocarpine hydrochloride Sigma-Aldrich Cat#P6503-5G Phosphate buffered saline Sigma-Aldrich Cat#SIAL-P4417-100TAB Paraformaldehyde VWR International Cat#100504-858 Sodium azide Sigma-Aldrich Cat#S2002-100G Triton X-100 Sigma-Aldrich Cat#X100-100ml Normal goat serum Fisher Scientific Cat#NC9660079 ProLong Gold with DAPI Life Technologies Cat#P36931 Vybrant DiI Cell-Labeling Solution Invitrogen Cat#V22885 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory JAX Strain #:000664; RRID: IMSR_JAX:000664 Mouse: Scn2a+/- Planells-Cases et al.80 N/A Mouse: Alpha6 - Cre Mutant Mouse Recourse & Research Centers RRID: MMRRC_015966-UCD Mouse: Scn2a+/fl Spratt et al.16 N/A Software and algorithms IGOR Pro Wavemetrics RRID: SCR_000325; v6.3 Prism Graphpad RRID: SCR_002798; v9 LabView NI RRID: SCR_014325; v2020 MATLAB MathWorks RRID: SCR_001622; vR2020a DeepLabCut Mathis et al.81 RRID: SCR_021391; v2.2.0.5 KiloSort2 Pachitariu et al.82,83 RRID: SCR_016422; https://github.com/ MouseLand/Kilosort Phy Cyrille Rossant https://github.com/cortex-lab/phy Mouse eye tracking acquisition code in LabView Liu et al.84; Bouvier et al.83 N/A Visual stimuli code in MATLAB Liu et al.84 N/A Human eye tracking acquisition code Dave Jones https://github.com/waveform80/picamera Human motion tracking (IMU) acquisition code This paper https://doi.org/10.5281/zenodo.10573756 Neuron 112, 1–12.e1–e5, May 1, 2024 e1 ..
Article Title: Vascular Compartmentalization of Functional Hyperemia from the Synapse to the Pia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Hoescht Life Technologies Cat#3342 Olig-2 rabbit Millipore Cat#AB9610 GFP chicken Invitrogen Cat#A10262 PDGFaR rat BD Biosciences Cat#558774 Anti-rabbit 550 Cliniscience Cat#H-1000 Anti-rabbit 405 Invitrogen Cat#A-31556 Anti-rat 633 Invitrogen Cat#A-21094 Anti-chicken 488 Invitrogen Cat#A11039 Chemicals, Peptides, and Recombinant Proteins Texas Red Dextran (70 kDa) Molecular Probes, Thermo Fisher Scientific Cat#D1830 Medetomidine Orion Pharma Domitor Ketamine Virbac Ketamine 1000 Xylazine Bayer Rompun 2% CaRuby-Nano Dextran (6 kDa) Collot et al., 2015 (Jean-Maurice Mallet) N/A Clacium Green-1 Dextran (10 kDa) Invitrogen Cat#C3713 Tamoxifen Sigma Cat#T5648 Paraformaldehyde Electron Microscopy Sciences Cat#15710 Normal Goat Serum Blocking Solution Cliniscience Cat#S-1000 Vecatashield Cliniscience Cat#H-1000 Texas Red-BSA Invitrogen Cat#A23017 Gelatin Sigma Cat#G1890 Triton X-100 Sigma Cat#T9284 Texas Red Dextran (3000 MW) Invitrogen Cat#D3329 D-AP5 Hello Bio Cat#HB0225 NBQX Hello Bio Cat#HB0443 MPEP Tocris Cat#1212 CPCCOEt Tocris Cat1028 LY 341495 Tocris Cat#1209 Experimental Models: Organisms/Strains Mouse: NG2-CreERT2 Huang et al., 2014 (Frank Kirchhoff) N/A Mouse: Ai95(RCL-GCaMP6f)-D Madisen et al., 2015 (Hongkui Zeng, Allan Institute) See Jax Stock No: 024105 Mouse: C57BL/6 Janvier Labs SSAL (Orléans) 1993 (F172) Mouse: C57BL/6J-Tg(Thy1-GCaMP6f) GP5.11Dkim/J The Jackson Laboratory Stock No: 025339 Software and Algorithms MATLAB 2013 MathWorks https://www.mathworks.com/ ImageJ NIH https://imagej.nih.gov/ij/ Prism GraphPad https://www.graphpad.com/scientific- software/prism/ LabView 2013 National Instruments http://www.ni.com/fr-fr/shop/labview.html Clampfit 10.7 Axon Instruments https://www.moleculardevices.com/ products/axon-patch-clamp-system e1 Neuron 99, 362–375.e1–e4, July 25, 2018 ..
Saline:Article Title: Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP Rockland Immunochemicals Cat#600-401-379S; RRID: AB_11182807 Chicken anti-GFP Aves Labs Cat#GFP-1010; RRID: AB_2307313 Guinea pig anti-VGlut Millipore Cat#AB5905; RRID: AB_2301751 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat#A-11011 Alexa Fluor 488 goat anti-chicken Invitrogen Cat#A-11039 Alexa Fluor 568 goat anti-guinea pig Invitrogen Cat#A-11075 Bacterial and virus strains AAV-EF1ɑ-Cre-mCherry UNC Viral Core N/A AAV-PhP.eb-CMV-sadCas9-VP64 Tamura et al.52 N/A AAV-PhP.eb-U6-sasgRNA-CMV-mCherry Tamura et al.52 N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris Cat#2312 Picrotoxin Tocris Cat#1128 CPCCOEt Tocris Cat1028 Pilocarpine hydrochloride Sigma-Aldrich Cat#P6503-5G Phosphate buffered saline Sigma-Aldrich Cat#SIAL-P4417-100TAB Paraformaldehyde VWR International Cat#100504-858 Sodium azide Sigma-Aldrich Cat#S2002-100G Triton X-100 Sigma-Aldrich Cat#X100-100ml Normal goat serum Fisher Scientific Cat#NC9660079 ProLong Gold with DAPI Life Technologies Cat#P36931 Vybrant DiI Cell-Labeling Solution Invitrogen Cat#V22885 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory JAX Strain #:000664; RRID: IMSR_JAX:000664 Mouse: Scn2a+/- Planells-Cases et al.80 N/A Mouse: Alpha6 - Cre Mutant Mouse Recourse & Research Centers RRID: MMRRC_015966-UCD Mouse: Scn2a+/fl Spratt et al.16 N/A Software and algorithms IGOR Pro Wavemetrics RRID: SCR_000325; v6.3 Prism Graphpad RRID: SCR_002798; v9 LabView NI RRID: SCR_014325; v2020 MATLAB MathWorks RRID: SCR_001622; vR2020a DeepLabCut Mathis et al.81 RRID: SCR_021391; v2.2.0.5 KiloSort2 Pachitariu et al.82,83 RRID: SCR_016422; https://github.com/ MouseLand/Kilosort Phy Cyrille Rossant https://github.com/cortex-lab/phy Mouse eye tracking acquisition code in LabView Liu et al.84; Bouvier et al.83 N/A Visual stimuli code in MATLAB Liu et al.84 N/A Human eye tracking acquisition code Dave Jones https://github.com/waveform80/picamera Human motion tracking (IMU) acquisition code This paper https://doi.org/10.5281/zenodo.10573756 Neuron 112, 1–12.e1–e5, May 1, 2024 e1 ..
Mutagenesis:Article Title: Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP Rockland Immunochemicals Cat#600-401-379S; RRID: AB_11182807 Chicken anti-GFP Aves Labs Cat#GFP-1010; RRID: AB_2307313 Guinea pig anti-VGlut Millipore Cat#AB5905; RRID: AB_2301751 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat#A-11011 Alexa Fluor 488 goat anti-chicken Invitrogen Cat#A-11039 Alexa Fluor 568 goat anti-guinea pig Invitrogen Cat#A-11075 Bacterial and virus strains AAV-EF1ɑ-Cre-mCherry UNC Viral Core N/A AAV-PhP.eb-CMV-sadCas9-VP64 Tamura et al.52 N/A AAV-PhP.eb-U6-sasgRNA-CMV-mCherry Tamura et al.52 N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris Cat#2312 Picrotoxin Tocris Cat#1128 CPCCOEt Tocris Cat1028 Pilocarpine hydrochloride Sigma-Aldrich Cat#P6503-5G Phosphate buffered saline Sigma-Aldrich Cat#SIAL-P4417-100TAB Paraformaldehyde VWR International Cat#100504-858 Sodium azide Sigma-Aldrich Cat#S2002-100G Triton X-100 Sigma-Aldrich Cat#X100-100ml Normal goat serum Fisher Scientific Cat#NC9660079 ProLong Gold with DAPI Life Technologies Cat#P36931 Vybrant DiI Cell-Labeling Solution Invitrogen Cat#V22885 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory JAX Strain #:000664; RRID: IMSR_JAX:000664 Mouse: Scn2a+/- Planells-Cases et al.80 N/A Mouse: Alpha6 - Cre Mutant Mouse Recourse & Research Centers RRID: MMRRC_015966-UCD Mouse: Scn2a+/fl Spratt et al.16 N/A Software and algorithms IGOR Pro Wavemetrics RRID: SCR_000325; v6.3 Prism Graphpad RRID: SCR_002798; v9 LabView NI RRID: SCR_014325; v2020 MATLAB MathWorks RRID: SCR_001622; vR2020a DeepLabCut Mathis et al.81 RRID: SCR_021391; v2.2.0.5 KiloSort2 Pachitariu et al.82,83 RRID: SCR_016422; https://github.com/ MouseLand/Kilosort Phy Cyrille Rossant https://github.com/cortex-lab/phy Mouse eye tracking acquisition code in LabView Liu et al.84; Bouvier et al.83 N/A Visual stimuli code in MATLAB Liu et al.84 N/A Human eye tracking acquisition code Dave Jones https://github.com/waveform80/picamera Human motion tracking (IMU) acquisition code This paper https://doi.org/10.5281/zenodo.10573756 Neuron 112, 1–12.e1–e5, May 1, 2024 e1 ..
Software:Article Title: Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP Rockland Immunochemicals Cat#600-401-379S; RRID: AB_11182807 Chicken anti-GFP Aves Labs Cat#GFP-1010; RRID: AB_2307313 Guinea pig anti-VGlut Millipore Cat#AB5905; RRID: AB_2301751 Alexa Fluor 568 goat anti-rabbit Invitrogen Cat#A-11011 Alexa Fluor 488 goat anti-chicken Invitrogen Cat#A-11039 Alexa Fluor 568 goat anti-guinea pig Invitrogen Cat#A-11075 Bacterial and virus strains AAV-EF1ɑ-Cre-mCherry UNC Viral Core N/A AAV-PhP.eb-CMV-sadCas9-VP64 Tamura et al.52 N/A AAV-PhP.eb-U6-sasgRNA-CMV-mCherry Tamura et al.52 N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris Cat#2312 Picrotoxin Tocris Cat#1128 CPCCOEt Tocris Cat1028 Pilocarpine hydrochloride Sigma-Aldrich Cat#P6503-5G Phosphate buffered saline Sigma-Aldrich Cat#SIAL-P4417-100TAB Paraformaldehyde VWR International Cat#100504-858 Sodium azide Sigma-Aldrich Cat#S2002-100G Triton X-100 Sigma-Aldrich Cat#X100-100ml Normal goat serum Fisher Scientific Cat#NC9660079 ProLong Gold with DAPI Life Technologies Cat#P36931 Vybrant DiI Cell-Labeling Solution Invitrogen Cat#V22885 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory JAX Strain #:000664; RRID: IMSR_JAX:000664 Mouse: Scn2a+/- Planells-Cases et al.80 N/A Mouse: Alpha6 - Cre Mutant Mouse Recourse & Research Centers RRID: MMRRC_015966-UCD Mouse: Scn2a+/fl Spratt et al.16 N/A Software and algorithms IGOR Pro Wavemetrics RRID: SCR_000325; v6.3 Prism Graphpad RRID: SCR_002798; v9 LabView NI RRID: SCR_014325; v2020 MATLAB MathWorks RRID: SCR_001622; vR2020a DeepLabCut Mathis et al.81 RRID: SCR_021391; v2.2.0.5 KiloSort2 Pachitariu et al.82,83 RRID: SCR_016422; https://github.com/ MouseLand/Kilosort Phy Cyrille Rossant https://github.com/cortex-lab/phy Mouse eye tracking acquisition code in LabView Liu et al.84; Bouvier et al.83 N/A Visual stimuli code in MATLAB Liu et al.84 N/A Human eye tracking acquisition code Dave Jones https://github.com/waveform80/picamera Human motion tracking (IMU) acquisition code This paper https://doi.org/10.5281/zenodo.10573756 Neuron 112, 1–12.e1–e5, May 1, 2024 e1 ..
Article Title: Vascular Compartmentalization of Functional Hyperemia from the Synapse to the Pia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Hoescht Life Technologies Cat#3342 Olig-2 rabbit Millipore Cat#AB9610 GFP chicken Invitrogen Cat#A10262 PDGFaR rat BD Biosciences Cat#558774 Anti-rabbit 550 Cliniscience Cat#H-1000 Anti-rabbit 405 Invitrogen Cat#A-31556 Anti-rat 633 Invitrogen Cat#A-21094 Anti-chicken 488 Invitrogen Cat#A11039 Chemicals, Peptides, and Recombinant Proteins Texas Red Dextran (70 kDa) Molecular Probes, Thermo Fisher Scientific Cat#D1830 Medetomidine Orion Pharma Domitor Ketamine Virbac Ketamine 1000 Xylazine Bayer Rompun 2% CaRuby-Nano Dextran (6 kDa) Collot et al., 2015 (Jean-Maurice Mallet) N/A Clacium Green-1 Dextran (10 kDa) Invitrogen Cat#C3713 Tamoxifen Sigma Cat#T5648 Paraformaldehyde Electron Microscopy Sciences Cat#15710 Normal Goat Serum Blocking Solution Cliniscience Cat#S-1000 Vecatashield Cliniscience Cat#H-1000 Texas Red-BSA Invitrogen Cat#A23017 Gelatin Sigma Cat#G1890 Triton X-100 Sigma Cat#T9284 Texas Red Dextran (3000 MW) Invitrogen Cat#D3329 D-AP5 Hello Bio Cat#HB0225 NBQX Hello Bio Cat#HB0443 MPEP Tocris Cat#1212 CPCCOEt Tocris Cat1028 LY 341495 Tocris Cat#1209 Experimental Models: Organisms/Strains Mouse: NG2-CreERT2 Huang et al., 2014 (Frank Kirchhoff) N/A Mouse: Ai95(RCL-GCaMP6f)-D Madisen et al., 2015 (Hongkui Zeng, Allan Institute) See Jax Stock No: 024105 Mouse: C57BL/6 Janvier Labs SSAL (Orléans) 1993 (F172) Mouse: C57BL/6J-Tg(Thy1-GCaMP6f) GP5.11Dkim/J The Jackson Laboratory Stock No: 025339 Software and Algorithms MATLAB 2013 MathWorks https://www.mathworks.com/ ImageJ NIH https://imagej.nih.gov/ij/ Prism GraphPad https://www.graphpad.com/scientific- software/prism/ LabView 2013 National Instruments http://www.ni.com/fr-fr/shop/labview.html Clampfit 10.7 Axon Instruments https://www.moleculardevices.com/ products/axon-patch-clamp-system e1 Neuron 99, 362–375.e1–e4, July 25, 2018 ..
Electron Microscopy:Article Title: Vascular Compartmentalization of Functional Hyperemia from the Synapse to the Pia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Hoescht Life Technologies Cat#3342 Olig-2 rabbit Millipore Cat#AB9610 GFP chicken Invitrogen Cat#A10262 PDGFaR rat BD Biosciences Cat#558774 Anti-rabbit 550 Cliniscience Cat#H-1000 Anti-rabbit 405 Invitrogen Cat#A-31556 Anti-rat 633 Invitrogen Cat#A-21094 Anti-chicken 488 Invitrogen Cat#A11039 Chemicals, Peptides, and Recombinant Proteins Texas Red Dextran (70 kDa) Molecular Probes, Thermo Fisher Scientific Cat#D1830 Medetomidine Orion Pharma Domitor Ketamine Virbac Ketamine 1000 Xylazine Bayer Rompun 2% CaRuby-Nano Dextran (6 kDa) Collot et al., 2015 (Jean-Maurice Mallet) N/A Clacium Green-1 Dextran (10 kDa) Invitrogen Cat#C3713 Tamoxifen Sigma Cat#T5648 Paraformaldehyde Electron Microscopy Sciences Cat#15710 Normal Goat Serum Blocking Solution Cliniscience Cat#S-1000 Vecatashield Cliniscience Cat#H-1000 Texas Red-BSA Invitrogen Cat#A23017 Gelatin Sigma Cat#G1890 Triton X-100 Sigma Cat#T9284 Texas Red Dextran (3000 MW) Invitrogen Cat#D3329 D-AP5 Hello Bio Cat#HB0225 NBQX Hello Bio Cat#HB0443 MPEP Tocris Cat#1212 CPCCOEt Tocris Cat1028 LY 341495 Tocris Cat#1209 Experimental Models: Organisms/Strains Mouse: NG2-CreERT2 Huang et al., 2014 (Frank Kirchhoff) N/A Mouse: Ai95(RCL-GCaMP6f)-D Madisen et al., 2015 (Hongkui Zeng, Allan Institute) See Jax Stock No: 024105 Mouse: C57BL/6 Janvier Labs SSAL (Orléans) 1993 (F172) Mouse: C57BL/6J-Tg(Thy1-GCaMP6f) GP5.11Dkim/J The Jackson Laboratory Stock No: 025339 Software and Algorithms MATLAB 2013 MathWorks https://www.mathworks.com/ ImageJ NIH https://imagej.nih.gov/ij/ Prism GraphPad https://www.graphpad.com/scientific- software/prism/ LabView 2013 National Instruments http://www.ni.com/fr-fr/shop/labview.html Clampfit 10.7 Axon Instruments https://www.moleculardevices.com/ products/axon-patch-clamp-system e1 Neuron 99, 362–375.e1–e4, July 25, 2018 ..
Blocking Assay:Article Title: Vascular Compartmentalization of Functional Hyperemia from the Synapse to the Pia
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Hoescht Life Technologies Cat#3342 Olig-2 rabbit Millipore Cat#AB9610 GFP chicken Invitrogen Cat#A10262 PDGFaR rat BD Biosciences Cat#558774 Anti-rabbit 550 Cliniscience Cat#H-1000 Anti-rabbit 405 Invitrogen Cat#A-31556 Anti-rat 633 Invitrogen Cat#A-21094 Anti-chicken 488 Invitrogen Cat#A11039 Chemicals, Peptides, and Recombinant Proteins Texas Red Dextran (70 kDa) Molecular Probes, Thermo Fisher Scientific Cat#D1830 Medetomidine Orion Pharma Domitor Ketamine Virbac Ketamine 1000 Xylazine Bayer Rompun 2% CaRuby-Nano Dextran (6 kDa) Collot et al., 2015 (Jean-Maurice Mallet) N/A Clacium Green-1 Dextran (10 kDa) Invitrogen Cat#C3713 Tamoxifen Sigma Cat#T5648 Paraformaldehyde Electron Microscopy Sciences Cat#15710 Normal Goat Serum Blocking Solution Cliniscience Cat#S-1000 Vecatashield Cliniscience Cat#H-1000 Texas Red-BSA Invitrogen Cat#A23017 Gelatin Sigma Cat#G1890 Triton X-100 Sigma Cat#T9284 Texas Red Dextran (3000 MW) Invitrogen Cat#D3329 D-AP5 Hello Bio Cat#HB0225 NBQX Hello Bio Cat#HB0443 MPEP Tocris Cat#1212 CPCCOEt Tocris Cat1028 LY 341495 Tocris Cat#1209 Experimental Models: Organisms/Strains Mouse: NG2-CreERT2 Huang et al., 2014 (Frank Kirchhoff) N/A Mouse: Ai95(RCL-GCaMP6f)-D Madisen et al., 2015 (Hongkui Zeng, Allan Institute) See Jax Stock No: 024105 Mouse: C57BL/6 Janvier Labs SSAL (Orléans) 1993 (F172) Mouse: C57BL/6J-Tg(Thy1-GCaMP6f) GP5.11Dkim/J The Jackson Laboratory Stock No: 025339 Software and Algorithms MATLAB 2013 MathWorks https://www.mathworks.com/ ImageJ NIH https://imagej.nih.gov/ij/ Prism GraphPad https://www.graphpad.com/scientific- software/prism/ LabView 2013 National Instruments http://www.ni.com/fr-fr/shop/labview.html Clampfit 10.7 Axon Instruments https://www.moleculardevices.com/ products/axon-patch-clamp-system e1 Neuron 99, 362–375.e1–e4, July 25, 2018 ..
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